Cytochrome P450 Reductase (CPR) Rat ELISA Kit

Product Quantity: 100 assays Manufacturer: Biovision

8,650,625.00 £

Alternative names:

NADPH-cytochrome P450 reductase (CPR, EC 1.6.2.4) is a ~78 kDa membrane-bound flavoenzyme that catalyzes the transfer of electrons from NADPH to members of the cytochrome P450 monooxidase (CYP) enzyme family in the endoplasmic reticulum. As CPR is required for the function of all CYP isozymes, it plays a critical role in the metabolism of drugs, organic pollutants and other xenobiotic compounds, in addition to its role in biosynthesis of certain vitamins and steroid hormones. BioVision’s rat CPR ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat CPR-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to CPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain CPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the rat CPR captured onto the plate. The concentration of CPR in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 0.312 ng/ml – 20 ng/ml. Sensitivity: 0.312 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%. Shipping Conditions:  gel pack

Storage Conditions: -20 Celsius

Shelf life: 12 months

Kit components: • Rat CPR Ab coated plate • Rat CPR standard • Detection Reagent A • Detection Reagent B • Standard diluent • Wash Buffer (30X) • Assay diluent A • Assay diluent B • TMB • Stop solution • Plate Sealers

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- Rat • Application- Quantitative protein detection, measure expression of Cytochrome Reductase after induction, compare CPR content in healthy versus diseased or drug-treated samples

Detection Method: Absorbance (450 nm)

Species Reactivity: Rat

Applications: Quantitative protein detection, measure expression of Cytochrome Reductase after induction, compare CPR content in healthy versus diseased or drug-treated samples

Sample type: • Tissue or cell lysates • Microsomes from rat tissues or cells

Features & Benefits: • Easy, convenient and time-saving method to measure rat cytochrome P450 reductase in a variety of samples • Sensitivity: 0.312 ng/ml (Detection Range: 0.312ng/ml – 20 ng/ml)

Used for studies on: Cytochrome P450 Reductase ELISA, CPR ELISA

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For more information, please, refer to the full datasheet.