Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
- Lysate Type: Tissue
- Tested Applications: WB
- Applications: N/A
- Physical State: Liquid
- Buffer: Colon Tissue Lysate (Normal)is supplied in SDS sample buffer containing 5% β
- -mercaptoethanol.
- Concentration: 2 mg/mL
- Source: Human Colon Tissue
- Tissue by diagnosis: Normal
- Tissue by development stage: N/A
- Tissue by species: Human
- Tissue cell/type: Colon
- Diagnosis: Normal
- Cancer type: N/A
- Cancer extended: N/A
- Age: 79
- Sex: M
- Race: Unknown
- Species: Human
- Growth Media: N/A
This product is intended for laboratory research purposes only and should be used by qualified personnel only. ProSci lysates are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci lysates.
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