A sandwich CLIA kit for quantitative measurement of Human E-Cad (E-Cadherin) in samples from Serum, Plasma, Cell supernatant
Gentaur's E-Cad CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human E-Cad , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human E-Cad . You can calculate the concentration of Human E-Cad in the samples by comparing the RLU value of the samples to the standard curve.
The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.
- Species reactivity: Human
- Antigen: E-Cad
- Assay type: Sandwich CLIA
- Sensitivity: 18.75pg/mL
- Detections range: from: 31.25 to: 2000pg/mL
- Validated sample types: Serum, Plasma, Cell supernatant
This Human E-Cad CLIA kit is intended for research use only. Do not use for human or animal diagnostics.