ChAT Peptide

(CT) Choline acetyltransferase Peptide

Store at -20°C. When reconstituted minimize freezing and thawing.

• Tested Applications: Bl
• Applications: ChAT Peptide is used for blocking the activity of ChAT antibody (catalog number 45-038). The ChAT antibody ships at 0.5 mg/mL and the ChAT Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
• Physical state: Lyophilized
• Buffer: ChAT peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
• Concentration:
• NCBI official symbol: CHAT
• NCBI gene ID#: 1103
• NCBI official full name: choline O-acetyltransferase
• NCBI organism: Homo sapiens
• SWISSPROT #: P28329
• Background Reference 1:
• Background Reference 2:
• Background Reference 3:
• Species: Human
• Location: ChAT peptide corresponds to 14 amino acids near the C-terminus region of ChAT.

This product is for research use only.