Caspase-8 Antibody

Product Quantity: 100 ug Manufacturer: Biovision

407.75 £

Antibody Target: Caspase-8

Target Alternative Name: CASP8 , MGC78473 , CASP-8, MACH, procaspase-8, MCH5, ALPS2B, FLICE, CAP4, EC 3.4.22.61

Tagline: 

Category: Caspase

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Clone: 

Species Reactivity: mouse, rat, bovine, monkey

Immunogen Sequence: Synthetic peptide surrounding amino acid 392 of mouse caspase-8

Accession: BC006737

Gene ID: 12370

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.2 mg/ml

Formulation: 100 ug (0.2 mg/ml) protein A purified rabbit anti-caspase-8 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control: 

Application & Usage: Western blotting (0.5-4 ug/ml). The immunoaffinity purified antibody detects the full length (55 kDa), intermediate cleavage fragments (26-31 kDa) and cleaved large fragment (18 kDa) of caspase-8.

Background Descriptions: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Not Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.