Caspase-2 Antibody

Product Quantity: 100 ug Manufacturer: Biovision

380.75 £

Antibody Target: Caspase-2

Target Alternative Name: CASP-2, NEDD-2,NEDD2, ICH1, ICH-1L/1S, ICH-1L, EC 3.4.22.55

Tagline: 

Category: Caspase

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Clone: 

Species Reactivity: Human, mouse, rat

Immunogen Sequence: Synthetic peptide surrounding amino acid 417 of mouse caspase-2

Accession: D28492

Gene ID: 12366

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.2 mg/ml

Formulation: 100 ug (0.2 mg/ml) affinity purified rabbit anti-caspase-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal

Purification: Affinity purified

Application: Western blot, Immunohistochemistry

Positive Control: Western Blot: Jurkat cell lysate, treated and untreated. IHC: Placenta tissue.

Application & Usage: Western blotting (1-2 ug/ml), and Immunohistochemistry (5 ug/ml). However, the optimal conditions should be determined individually. Detects the full length and small subunit of caspase-2. In human samples, it also detects a 30 kDa intermeadiate fragment

Background Descriptions: Caspases play a key role in apoptosis. Similar as other caspases, caspase-2 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Caspase-2 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named ICH-1S. Over expression of ICH-1L induces apoptosis, while over expression of ICH-1S suppresses Rat-1 cell death induced by serum deprivation.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.