Bid Antibody

Product Quantity: 100 ug Manufacturer: Biovision

380.75 £

Antibody Target: Bid

Target Alternative Name: FP497 , MGC42355, MGC15319

Tagline: 

Category: Bcl-2

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Clone: 

Species Reactivity: Mouse

Immunogen Sequence: Synthetic peptide surrounding amino acid 45 of mouse Bid

Accession: P70444

Gene ID: 12122

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 ug (0.5 mg/ml) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control: 

Application & Usage: Western blot analysis (0.5-5 ug/ml) also works well in immunoprecipitation (20 ug/ml) and Immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the full length (22 kDa) mouse Bid. Reactivity to other species has not been tested.

Background Descriptions: Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Not Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.