Biomolecule/Target: BD-4
Alternates names: Beta-defensin 3, Defensin, beta 103, Defensin-like protein, DEFB103A, BD3, DEFB103, DEFB3, DEFB103B
Synonyms: DEFB104A; DEFB4; BD-4; DEFB-4; DEFB104; MGC118942; MGC118944; MGC118945; hBD-4
Country of Animal Origin: USA
NCBI Gene ID: 140596
NCBI Gene Symbol: DEFB4
Gene Source: Human
Accession: Q8WTQ1
Recombinant: Yes
Source: E. coli
Purity Determined by SDS-PAGE: >=98%
Purity Determined by HPLC: >=98%
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological acivity: Determined by its ability to chemoattract human monocytes using a concentration range of 0.1-50 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: ~6.0 kDa
Storage Temperature: -20 Celsius
Shipping: Gel pack
Shelf Life: 12 months
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered and lyophilized from 20 mM PBS, pH 7.4 and 130 mM NaCl.
Reconstitution Instructions: Centrifuge the Vial prior to opening. Reconstitute in sterile dd H2O to a concentration >= 100 ug/ml. This solution can then be diluted into other aqueous buffers and stored at 4 Celsius for 2-7 days and at -20 Celsius for future use.
Background Information: Defensins are cationic peptides with a large spectrum of antimicrobial actIVity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. Four human Beta-defensins haVe been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial actIVities, they are chemoattractant towards immature dendritic cells and memory T cells. Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleaVage of a signal sequence (for BD-1 a propeptide region). Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta Defensin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 50 amino acids. The BD-4 is purified by proprietary chromatographic techniques.
Amino acid sequence: N/A
Handling: In order to retain the quality of the proteins, please, aVoid cycles of freezing and thawing. For proteins that are in liquid form or reconstituted lyophilized proteins small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the Vial to gather all the solution on the bottom.
Usage: For Research Use Only! Not to be used in humans
Product type:
For more information, please, refer to the full datasheet.