BAG-1 Antibody: Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- BAG-1 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Apoptosis, Cancer
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: N/A
- Immunogen: BAG-1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human BAG-1.
- The immunogen is located within the first 50 amino acids of BAG-1.
- Tested Applications: E, WB, IHC-P, IF
- Applications: BAG-1 antibody can be used for the detection of BAG-1 by Western blot at 1 - 2 μ
- g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ
- g/mL. For immunofluorescence start at 20 μ
- g/mL.
- Antibody validated: Western Blot in human samples
- Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- Specificity: N/A
- Positive control 1: Cat. No. 1216 - PC-3 Cell Lysate
- Positive control 2: Cat. No. 1369 - Human Lymph node Tissue Lysate
- Positive control 3: Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: N/A
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: BAG-1 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: BAG-1 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: BAG1
- Accession #: NP_004314
- Protein GI #: 124494251
- NCBI gene ID#: 573
- NCBI official full name: BCL2-associated athanogene
- NCBI organism: Homo sapiens
- SWISSPROT #: Q99933
Optimal dilutions for each application to be determined by the researcher.
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