Alternative names:
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples. BioVision’s Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at ? = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: • Ascorbic Acid Assay Buffer • Ascorbic Acid Probe (in DMSO) • Catalyst • Ascorbic Acid Enzyme Mix (lyophilized) • Ascorbic Acid Standard (20 umole)
Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
Detection Method:
Species Reactivity:
Applications:
Sample type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, food samples, growth medium, etc.
Features & Benefits: • Simple procedure; takes ~ 5 minutes for the reaction to complete • Fast and convenient
Used for studies on: Ascorbic Acid Assay
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For more information, please, refer to the full datasheet.
