Biomolecule/Target:
Alternates names: Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP
Synonyms: Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP
Country of Animal Origin: USA
NCBI Gene ID:
NCBI Gene Symbol:
Gene Source: N/A
Accession:
Recombinant: No
Source: BoVine lung
Purity Determined by SDS-PAGE: >=98%
Purity Determined by HPLC: >=98%
Endotoxin Level: <0.1 ng/ug Activity (Specifications/test method): N/A
Biological acivity: 5 x 10^6 IU/mg.
Results: 6 x 10^6 IU/mg.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 6.5 kDa
Storage Temperature: -20 Celsius
Shipping: Gel pack
Shelf Life: 12 months
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered and lyophilized with no additIVes
Reconstitution Instructions: Reconstitute in H2O to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4 Celsius for 1 week or –20 Celsius for future use. For long-term storage, it is recommend to add a carrier protein (e.g., 0.1% BSA). PreVent freeze/thaw cycles.
Background Information: Aprotinin inhibits the actIVity of seVeral proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. It is present in blood and in most tissues, with a high concentration in lung, inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it preVents the expression of pro-inflammatory adhesIVe glycoproteins. Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids arranged in a single polypeptide chain, cross-linked by three disulfide bridges and haVing a molecular mass of 6512 Daltons. Aprotinin is purified by proprietary chromatographic techniques.
Amino acid sequence: N/A
Handling: In order to retain the quality of the proteins, please, aVoid cycles of freezing and thawing. For proteins that are in liquid form or reconstituted lyophilized proteins small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the Vial to gather all the solution on the bottom.
Usage: For Research Use Only! Not to be used in humans
Product type:
For more information, please, refer to the full datasheet.
