A polyclonal antibody for MELK from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic peptide of human MELK (AA249-263). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This MELK antibody is conjugated to Dylight 350.
MELK is a protein-serine/threonine kinase in the CAMK Ser/Thr protein kinase family, SNF1 subfamily. It is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It exhibits a broad substrate specificity with targets that include BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. It is an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1 Its phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. It inhibits spliceosome assembly during mitosis by phosphorylating ZNF622, which contributes to its redirection to the nucleus. It interacts with ZNF622 and PPP1R8. It is activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. It is up-regulated in many cancers cells. It is expressed in placenta, kidney, thymus, testis, ovary and intestine, and is up-regulated in many cancers cells. It can occur in 8 human isoforms ranging from 74.642 to 52.528 kDa in size.
- Shipped on ice packs at +4ºC. Upon receipt keep at -20ºC.
- The gross weight ( in kg) of the package is ~1.4 kg. The net weight of the product is ~0.1 grams.
- The item is not hazardous according to both ADR and UN. Its tariff code is 3002.15.0000 (Immunological products, put up in measured doses or in forms or packings for retail sale). The UNSPSC code of the product is 12352203 (Antibodies).
This product is not for human or animal treatment or consumption. Not for use in diagnostics or therapeutics. For in vitro research use only.
0 reviews for Stressmarq