Angiopoietin-1 (human) ELISA Kit

Product Quantity: 100 assays Manufacturer: Biovision

7,536,875.00 £

Alternative names: ANGPT1 (human) ELISA Kit

BioVision’s Human Angiopoietin-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-1. This assay employs an antibody specific for human Angiopoietin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1?, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF). The minimum detectable dose of Angiopoietin-1 is typically less than 30 pg/ml. Detection Range: 30 pg/ml – 18000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Shipping Conditions:  gel pack

Storage Conditions: -20 Celsius

Shelf life: 12 months

Kit components: • Angiopoietin-1 Microplate (Item A) coated with anti-human Angiopoietin-1, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human Angiopoietin-1) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) • Detection Antibody Angiopoietin-1 (Item F), biotinylated anti-human Angiopoietin-1 (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 400x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- human. • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

Detection Method: Absorbance (450 nm)

Species Reactivity: human

Applications: Application- Quantitative protein detection, establishing normal range, validation of antibody array results

Sample type: • Serum & plasma • Cell culture supernatants • Urine

Features & Benefits: • Easy, convenient and time-saving method to assay for human Angiopoietin-1. • The minimum detectable dose of Angiopoietin-1 is typically less than 30 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1?, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF).

Used for studies on: Angiopoietin-1

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For more information, please, refer to the full datasheet.