Alternative names: alpha-D-Glucosidase Activity Colorimetric Assay Kit , alpha-D-Glucoside Glucohydrolase Activity Colorimetric Assay Kit , alpha-Glucopyranosidase Activity Colorimetric Assay Kit, Maltase Activity Colorimetric Assay Kit, alpha-1,4-Glucosidase Activity Colorimetric Assay Kit, Glucoinvertase Activity Colorimetric Assay Kit
alpha-Glucosidase breaks down alpha-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, alpha-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to alpha-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms. In BioVision’s alpha-Glucosidase Activity Colorimetric Assay Kit, alpha-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of alpha-glucosidase activity in a variety of samples.
Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: • alpha-Glucosidase Assay Buffer • alpha-Glucosidase Substrate Mix • alpha-Glucosidase Positive Control • p-Nitrophenol Standard (100 mM)
Summary: • Detection method- Absorbance (OD 410 nm) • Applications: – Measurement of alpha-Glucosidase activity in biological samples – Screening alpha-Glucosidase inhibitors
Detection Method:
Species Reactivity:
Applications:
Sample type: • Serum • Saliva • Tissue • Cell Culture
Features & Benefits: • Rapid and easy-to-use. • Highly-sensitive & high-throughput capable • This assay kit can measure 0.1-10 mU of alpha-glucosidase activity in a variety of sample.
Used for studies on: alpha-Glucosidase
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For more information, please, refer to the full datasheet.