AlbuVoid Binding Buffer AVBB

Product Quantity: 1000 mL Manufacturer: Biotech Support Group

413.00 £

AlbuVoid – Albumin Depletion Plus Low Abundance Serum Protein Enrichment Albumin voids in flow-through >95%, with <30 minute bind/wash/elute protocol Low abundance enrichment equivalent or better than hexa-peptides or antibodies Disposable, cost-effective, no column regeneration or cross-contamination The mild elution procedure maintains the tertiary structure and makes samples purified with AlbuVoid perfect for transferring to secondary analysis The eluted fractions retain their enzymatic and biological activity AlbuVoid removes albumin from many species including human, mouse, sheep, bovine, goat, rat, calf, cell culture. The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies. AlbuVoid is an albumin depletion reagent kit. It removes albumin from serum and plasma samples while concentrating low abundance, and/or low molecular weight proteins. The AlbuVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions. AlbuVoid considerably enhances resolution of proteins below 50 kD, a limitation of alternate enrichment protocols. AlbuVoid does not bind albumin, but all other proteins (except albumin) in the sample bind to AlbuVoid and then you can elute off all the proteins without the albumin. Resulting in low abundance serum protein enrichment. It is ideal for applications involving biomarker discovery, enzyme assays, toxicological studies for new drugs, protein profiling using SELDI analysis, protein array pixelation, 1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS and cytokines research. AlbuVoid derives from a silica-based library of individual mixed-mode polymeric ligands. The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. Because of its specific binding properties, AlbuVoid depletes high abundance proteins in serum like albumin while improving the resolution of less abundant serum proteins. GENTAUR has AlbuVoid for albumin depletion plus low abundance serum protein enrichment. Both Albusorb and AlbuVoid are available in AlbuTrial kit. AlbuTrial kit includes 1 gm of Albusorb and 5 Preps of AlbuVoid with the respective buffers needed for the assays. For more information, please, refer to the full datasheet of AlbuVoid. (here) Manufacturer: Biotech Support Group