Alternative names: CFD (human) ELISA Kit, Complement Factor D (human) ELISA Kit, Properdin Factor D Esterase (human) ELISA Kit, C3 Proactivator Convertase (human) ELISA Kit
BioVision’s Human Adipsin/Factor D/CFD/Complement Factor D/Properdin Factor D Esterase/C3 Proactivator Convertase ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Adipsin. This assay employs an antibody specific for human Adipsin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adipsin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Adipsin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adipsin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1?, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of Adipsin is typically less than 4 pg/ml. Detection Range: 4 pg/ml – 6000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: • Adipsin Microplate (Item A) coated with anti-human Adipsin, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human Adipsin) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) • Detection Antibody Adipsin (Item F), biotinylated anti-human Adipsin (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 300x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid
Summary: • Detection method- Absorbance (450 nm) • Species reactivity- human • Application- Quantitative protein detection, establishing normal range, validation of antibody array results
Detection Method: Absorbance (450 nm)
Species Reactivity: human
Applications: Quantitative protein detection, establishing normal range, validation of antibody array results
Sample type: • Serum & plasma • Cell culture supernatants • Urine
Features & Benefits: • Easy, convenient and time-saving method to assay Adipsin/Factor D. • The minimum detectable concentration of Resistin is less than 4 pg/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1?, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP2, TNF-alpha, TNF-beta, TPO, VEGF.
Used for studies on: Adipsin/Factor D
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For more information, please, refer to the full datasheet.
