Aconitase (aconitate hydratase; EC 126.96.36.199) is an iron-sulfur protein containing an [Fe?S?]?? cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a ?max at 450nm.
Shipping Conditions: gel pack
Storage Conditions: 4 Celsius
Shelf life: 12 months
Kit components: • Assay Buffer • Substrate • Developer • Enzyme Mix (200µl) • Cysteine • (NH?)?Fe(SO?)? • Isocitrate Standard (10umol)
Summary: • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian
Sample type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.
Features & Benefits: • Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Aconitase activity in various biological samples.
Used for studies on: Aconitase
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For more information, please, refer to the full datasheet.