Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
Shipping Conditions: gel pack
Storage Conditions: -80 Celsius
Shelf life: 6 months
Kit components: • ASMase Assay Buffer I • ASMase Assay Buffer II • ASMase Substrate (Lyophilized) • ASMase Enzyme Mix I (Lyophilized) • ASMase Enzyme Mix II (Lyophilized) • Choline Standard (Lyophilized) • ASMase Positive Control (Lyophilized) • ASMase Probe (DMSO)
Summary: • Detection method- Absorbance (570 nm) • Applications – Measurement of ASMase activity in various tissues/cell extracts – Screening of ASMase inhibitors
Detection Method: Absorbance (570 nm)
Applications: • Measurement of ASMase activity in various tissues/cell extracts • Screening of ASMase inhibitors
Sample type: • Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells • Serum
Features & Benefits: • Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
Used for studies on: Acid sphingomyleinase
Key Words: acid sphingomyelinase activity assay kit, acid sphingomyelinase activity kit, acid sphingomyelinase assay kit, acid sphingomyelinase kit, assay acid sphingomyelinase activity, measure acid sphingomyelinase activity, detect acid sphingomyelinase activity, quantify acid sphingomyelinase activity, quantitate acid sphingomyelinase activity, acid sphingomyelinase activity measurement kit, acid sphingomyelinase activity detection kit, acid sphingomyelinase activity quantitation kit, asmase activity assay kit, asmase activity kit, asmase assay kit, asmase kit, assay asmase activity, measure asmase activity, detect asmase activity, quantify asmase activity, quantitate asmase activity, asmase activity measurement kit, asmase activity detection kit, asmase activity quantitation kit, acid sphingomyelinase, asmase, August-AK-14
For more information, please, refer to the full datasheet.