Acid Sphingomyelinase Activity Colorimetric Assay Kit

Product Quantity: 100 assays Manufacturer: Biovision

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Alternative names:

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Shipping Conditions:  gel pack

Storage Conditions: -80 Celsius

Shelf life: 6 months

Kit components: • ASMase Assay Buffer I • ASMase Assay Buffer II • ASMase Substrate (Lyophilized) • ASMase Enzyme Mix I (Lyophilized) • ASMase Enzyme Mix II (Lyophilized) • Choline Standard (Lyophilized) • ASMase Positive Control (Lyophilized) • ASMase Probe (DMSO)

Summary: • Detection method- Absorbance (570 nm) • Applications – Measurement of ASMase activity in various tissues/cell extracts – Screening of ASMase inhibitors

Detection Method: Absorbance (570 nm)

Species Reactivity: 

Applications: • Measurement of ASMase activity in various tissues/cell extracts • Screening of ASMase inhibitors

Sample type: • Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells • Serum

Features & Benefits: • Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Used for studies on: Acid sphingomyleinase

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For more information, please, refer to the full datasheet.