Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. BioVision’s Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (?max = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.
Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: • AP Assay Buffer • pNPP Substrate (10 TAB) • AP Enzyme • Stop Solution
Summary: • Detection method- Absorbance (405 nm) • Species reactivity- Mammalian • Applications- Phosphate concentrations between 1 um and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Sample type: Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features & Benefits: • The assay detects acid phosphatase (AP) activity conveniently in various biological samples
Used for studies on: Phosphate assay
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For more information, please, refer to the full datasheet.