Alternative names: AChE Activity Colorimetric Assay Kit, Acetylhydrolase Activity Colorimetric Assay Kit
Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. BioVision’s Acetylcholinesterase Activity Assay Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570 nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The intermediate reacts with a highly specific probe to generate color. This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples.
Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: • AChE Assay Buffer • AChE Probe (DMSO) • AChE Substrate (Lyophilized) • Choline Oxidase Enzyme Mix (Lyophilized) • Choline Standard (Lyophilized) • AChE Positive Control (Lyophilized)
Summary: • Detection: Absorbance (OD 570 nm) • Application: – Measurement of AChE activity in various tissues/cells – Screening of AChE inhibitors
Detection Method: Absorbance (OD 570 nm)
Applications: • Measurement of AChE activity in various tissues/cells • Screening of AChE inhibitors
Sample type: • Serum, plasma or blood. • Animal tissues: liver, heart, kidney, etc. • Cell culture: Adherent or suspension cells
Features & Benefits: • Rapid, simple & convenient • This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples
Used for studies on: Acetylcholinesterase
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For more information, please, refer to the full datasheet.