Alternative names:
Acetoacetate (AcAc), a beta-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AcAc can be enzymatically reduced to 3-beta-hydroxybutyrate (beta-HB), or decarboxylated producing acetone (CH3)2CO). Ketone bodies (betaHB: 78%; AcAc: 20% & CH3)2CO: 2%) are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Traditionally, AcAc levels have been qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore. BioVision’s Acetoacetate Assay Kit has adapted that principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine. In this non-enzymatic assay, AcAc reacts with a substrate to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-beta-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 um.
Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: • Acetoacetate Assay Buffer • Acetoacetate Standard (Lyophilized) • Acetoacetate Substrate
Summary: • Detection method- Absorbance (550 nm) • Applications: – Measurement of acetoacetate in various biological samples • Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.
Detection Method: Absorbance (550 nm)
Species Reactivity:
Applications: • Measurement of acetoacetate in various biological samples. • Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.
Sample type: • Serum, plasma, blood, urine & other body fluids
Features & Benefits: • Specific for acetoacetate and does not detect 3-beta-hydroxybutyrate • The assay can detect less than 25 um of acetoacetate in various sample types.
Used for studies on: Acetoacetate
Key Words: acetoacetate assay kit, acetoacetate assay, acetoacetate kit, assay acetoacetate, measure acetoacetate, quantitate acetoacetate, detect acetoacetate, acetoacetate quantitation kit, acetoacetate quantification kit, acetoacetate measurement kit, acetoacetate
For more information, please, refer to the full datasheet.
