Alternative names: 6-PGA assay kit
6-Phosphogluconate (6-PGA) is an intermediate of both Pentose Phosphate Pathway (PPP) and Entner-Doudoroff Pathway. It is produced by the hydrolysis of 6-Phosphogluconolactone, catalyzed by 6-Phosphogluconolactonase. In the Pentose Phosphate Pathway, 6-PGA is utilized by 6-Phosphogluconate Dehydrogenase to generate ribulose-5-Phosphate and NADPH. These products are important for nucleic acid synthesis and various anabolic processes. In Prokaryotes, 6-Phosphogluconate is the main metabolite of Entner-Doudoroff pathway, and is converted into Pyruvate using both 6-Phosphogluconate Dehydratase and 2-Keto-3-Deoxyphosphogluconate aldolase. Recent studies show that long-term exposure to glucose perturbs the Pentose Phosphate Pathway, causes significant accumulation of 6-Phosphogluconate and impairs beta cell function. Measurement of 6-Phosphogluconate levels therefore is important for evaluating Pentose Phosphate Pathway, developing therapeutic approaches for diabetes research, and analyzing the Entner-Doudoroff Pathway in bacteria. BioVision’s 6-Phosphogluconate assay kit can be used with a variety of sample types. In this assay, 6-Phosphogluconate is converted to Ribulose-5-Phosphate by 6-Phosphogluconate Dehydrogenase in the presence of NAD, to form NADH, which reduces a probe and generates strong absorbance at 450 nm. This 6-Phosphogluconate Assay Kit is simple, sensitive & easy to use and can detect 6-Phosphogluconate levels lower than 20 um.
Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: 6-PGA Assay Buffer 6-PGA Enzyme 6-PGA Substrate Mix 6-PGA Standard
Summary: • Detection method- Absorbance (OD= 450 nm). • Species reactivity- Mammalian • Applications- Measurement of 6-Phosphogluconic Acid in various tissues/cells. Analysis of Pentose Phosphate Pathway and Entner-Doudoroff Pathway.
Detection Method: Detection method- Absorbance (OD= 450 nm).
Species Reactivity: Mammalian
Applications: • Measurement of 6-Phosphogluconic Acid in various tissues/cells. • Analysis of Pentose Phosphate Pathway and Entner-Doudoroff Pathway.
Sample type: • Tissues: e.g. Liver, Kidney, Heart • Adherent or Suspension Cells: e.g. HeLa, Jurkat cells
Features & Benefits: • Simple, fast and convenient assay • Can measure 6-PGA levels as low as 20 um
Used for studies on: Metabolism
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For more information, please, refer to the full datasheet.