Alternative names: 2-Phosphoglyceric Acid Colorimetric/Fluorometric Assay Kit, 2PG Colorimetric/Fluorometric Assay Kit
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research. BioVision’s 2-Phosphoglycerate Assay kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Shipping Conditions: gel pack
Storage Conditions: -20 Celsius
Shelf life: 12 months
Kit components: • 2PG Assay Buffer • 2PG Probe (in DMSO) • 2PG Enzyme Mix (Lyophilized) • 2PG Converter (Lyophilized) • 2PG Developer (Lyophilized) • 2PG Standard (Lyophilized)
Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Applications: -Measurement of 2-Phosphoglycerate in various tissues/cells -Analysis of carbohydrate metabolism and cell signaling -Cancer research
Sample type: • Animal tissues: e.g. liver, muscle and heart, etc. • Cell culture: adherent or suspension cells
Features & Benefits: • Sensitive, fast, and easy-to-use. • This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Used for studies on: Metabolism/Cancer
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For more information, please, refer to the full datasheet.