The real time polymerase chain reaction is a laboratory technique based on the polymerase chain reaction (PCR). While in Conventional PCR the DNA amplification is observed only as the end product of the reaction, in Real Time PCR the amplification of the DNA molecule can be monitored in real-time. Real time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR). There are two most commonly spread methods for detecting the PCR products in real time PCR: A) Via non-specific fluorescent dyes that intercalate with any double-stranded DNA, B) Via DNA probes which are sequence-specific and are made of oligonucleotides labelled with a fluorescent reporter molecule(s). This method permits detection of PCR products only after the probe has hybridized with its complementary sequence. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines suggest that the abbreviation qPCR should refer to quantitative real time PCR and that RT qPCR be used for reverse transcriptionqPCR. The abbreviation “RT-PCR” commonly is used for reverse transcription polymerase chain reaction and not real-time PCR, however not all authors agree with this convention, therefore a careful examination of the terms should be done.
Compatibility: Our PCR kits are compatible with most of the cyclers (LightCycler1.0/2.0; ABI Prism®7000/7300/7500/7900/Step One Plus; iCycler iQ4/iQ5; Smart Cycler II; Bio-Rad CFX 96; Rotor Gene6000; Mx3000P/3005P; MJ-Option2/Chromo4; LightCycler®480, etc.). Please, either send an enquiry regarding the compatibility of the particular real Time PCR kit, or mention the model of your Real Time PCR cycler when placing the order.
Storage Conditions: Store all reagents of the 16S rRNA Gene of Common Animals Real Time PCR Kit at -20°C. Storage at +4°C is not recommended. All of the reagents in the kit can be used until the expiration date indicated on the box. Freeze and thaw cycles should be avoided as this will reduce the sensitivity of the kit. Up to 3 cycles of freezing and thawing will not influence significantly the performance of the kit. Cool all reagents during the working steps. Reaction mix should be stored in dark.
Shipping Conditions: The 16S rRNA Gene of Common Animals Real Time PCR Kit should be shipped on dry ice
Materials required but not provided: Biological cabinet Real time PCR system Vortex mixer Real time PCR reaction tubes/plates Cryo-container Pipets (0.5ul 1000ul) Sterile filter tips for micro pipets Sterile microtubes Disposable gloves, powderless Biohazard waste container Refrigerator and Freezer Tube racks Desktop microcentrifuge -All the materials that are not included in the kit may be purchased from GENTAUR