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Trough GeneRiver you can obtain various Conventional PCR, Real Time PCR, Reverse Transcriptase (RT-PCR), Multiplex PCR, Quantitative real time PCR (Q-RT PCR) ready to use PCR kits and positive controls for detection of various pathogens, parasites, genes, mutations, etc. Our Real Time PCR kits are compatible with most of the PCR cyclers available on the market (LightCycler1.0/2.0; ABI Prism®7000/7300/7500/7900/Step One Plus; iCycler iQ™4/iQ™5; Smart Cycler II; Bio-Rad CFX 96; Rotor Gene™6000; Mx3000P/3005P; MJ-Option2/Chromo4; LightCycler®480, etc.). However, note that in some cases there are more than one version of the PCR kits and different versions are compatible with different cyclers. Please, contact us with information regarding the model of your PCR cycler in order to avoid discrepancies between the version of the kit and the cycler that will be used.

 

 

Summary of the Real Time PCR technique:

The real time polymerase chain reaction is a laboratory technique based on the polymerase chain reaction (PCR). While in Conventional PCR the DNA amplification is observed only as the end product of the reaction, in Real Time PCR the amplification of the DNA molecule can be monitored in real-time. Real time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).

There are two most commonly spread methods for detecting the PCR products in real time PCR:

  1. A) Via non-specific fluorescent dyes that intercalate with any double-stranded DNA,
  2. B) Via DNA probes which are sequence-specific and are made of oligonucleotides labelled with a fluorescent reporter molecule(s). This method permits detection of PCR products only after the probe has hybridized with its complementary sequence.

 

The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines suggest that the abbreviation qPCR should refer to quantitative real time PCR and that RT qPCR be used for reverse transcription–qPCR. The abbreviation “RT-PCR” commonly is used for reverse transcription polymerase chain reaction and not real-time PCR, however not all authors agree with this convention, therefore a careful examination of the terms should be done.