What are Ras ELISA Kit?

Ras ELISA kit is specifically designed for the study of Ras activation and can be used to study novel signalling pathways for Ras activation. The kit can also be used as a diagnostic test to detect oncogenic Ras associated with malignancy.

Ras GTPase

What are Ras GTPase?

elisa kit Ras GTPase

Ras, which is derived from ‘rat sarcoma virus’, is a family of related proteins that are expressed in all animal cell lines and organs. All members of the Ras family of proteins belong to a class of proteins called small GTPases, and are involved in signal transmission within cells (cellular signal transduction). Ras is the prototypical member of the Ras protein superfamily, which are all related in their three-dimensional structure and regulate various cellular behaviours.

Suppliers Offering Ras Kits ELISA

abbexa

ABBEXA

BlueGene Supplier

ABCAM

cell

CELL BIOLABS

merck

Merck Millipore

Selected Products

Human H ras ELISA kit
E01H0273-96
BlueGene 1 plate of 96 wells

CONTACT

Contact us with any questions and we will answer them in less than 24 hours.

We’ve developed a series of ELISA kit to detect RAS (GTPase) proteins in humans. These kits have been developed to detect this protein, which is involved in several signalling pathways and scientific and political processes, in different types of media, including breast fluid, plasma, soot cell fluid, cell culture fluid, textiles and urine.

The Ras ELISA kit uses the competitive enzyme immunoassay technique. It is based on the use of a monoclonal antibody to Ras protein and a Ras-HRP protein conjugate. The test matrix and buffer are incubated with the Ras-HRP protein conjugate in a pre-reusable container for one hour. After the incubation period, samples are decanted and washed five times. Then the wells are incubated with a substrate for the HRP enzyme. The product of the enzyme-substrate reaction forms a blue complement. Finally, a water sample is added. Finally, a parachute solution is added to stop the reaction. The intensity of the colour is measured spectrophotometrically at 450 nm in a microplate reader. Microplate reader.

The colour intensity is inversely proportional to the concentration of the Ras protein. Ras ligands and Ras-HRP conjugate are mixed with Ras anti-protein. The intensity of the colour is inversely proportional to the concentration of the Ras protein. Since the number of sites is limited, the more sites are occupied by the machine Ras protein, the fewer sites are available for binding to the conjugated Ras-HRP protein. A linear curve relates the colour intensity (D.O.) to the concentration of the parameters. The concentration of Ras protein in the sample.

The concentration of Ras protein in each sample is calculated from this curve. Principle of the test This kit is based on sandwich immunoassay technology. An antibody is pre-coated on a 96-well plate. Standards, test samples and biotin conjugate reagent are added to the wells and incubated. HRP conjugate reagent is then added and the entire plate is incubated. Unbound conjugates are removed with Wash Buffer in each step. TMB substrate is used to quantify the HRP enzyme reaction.

After addition of the TMB substrate, only wells with sufficient RRAS2 produce a blue coloured product, which turns yellow after addition of the acid stop solution. The intensity of the yellow colour is proportional to the amount of RRAS2 bound to the plate. The optical density (OD) is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of RRAS2 can be calculated.

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