Alternative names:
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe?S?]?? cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a ?max at 450nm.
Shipping Conditions: gel pack
Storage Conditions: 4 Celsius
Shelf life: 12 months
Kit components: • Assay Buffer • Substrate • Developer • Enzyme Mix (200µl) • Cysteine • (NH?)?Fe(SO?)? • Isocitrate Standard (10umol)
Summary: • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian
Detection Method:
Species Reactivity:
Applications:
Sample type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.
Features & Benefits: • Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Aconitase activity in various biological samples.
Used for studies on: Aconitase
Key Words: aconitase activity assay kit, aconitase activity kit, aconitase assay kit, aconitase kit, measure aconitase activity, quantitate aconitase activity, assay aconitase activity, aconitase activity, aconitase activity colorimetric assay kit, aconitase, glyoxylate, glyoxylate pathway
For more information, please, refer to the full datasheet.
